Neuron
Volume 9, Issue 1, July 1992, Pages 85-95
Journal home page for Neuron

Article
P-type calcium channels in rat central and peripheral neurons

https://doi.org/10.1016/0896-6273(92)90223-ZGet rights and content

Abstract

The peptide toxin ω-Aga-IVA blocked P-type Ca2+ channel current in rat Purkinje neurons (KD ≈ 2 nM) but had no effect on identified T-type, L-type, or N-type currents in a variety of central and peripheral neurons. ω-Aga-IVA blocked a substantial fraction of high threshold Ca2+ channel current in neurons from the hippocampal CA1 region (mean 26%), visual cortex (32%), spinal cord (45%), and dorsal root ganglia (23%), but less in hippocampal CA3 neurons (14%) and none in sympathetic neurons. In all cases, ω-Aga-IVA block could be reversed by a brief train of strong depolarizations. There was no overlap between current blocked by ω-Aga-IVA and the fractions blocked by dihydropyridines and ω-conotoxin GVIA, but not all current resistant to dihydropyridines and ω-conotoxin was blocked by ω-Aga-IVA. The results suggest that ω-Aga-IVA is highly selective for P-type channels and that many central neurons and some peripheral neurons possess substantial P-type current.

References (43)

  • M.E. Williams et al.

    Structure and functional expression of α1 α2, and β subunits of a novel human neuronal calcium channel subtype

    Neuron

    (1992)
  • B.P. Bean

    Nitrendipine block of cardiac calcium channels: high-affinity binding to the inactivated state

    Proc. Natl. Acad. Sci. USA

    (1984)
  • B.P. Bean

    Classes of calcium channels invertebrate cells

    Annu. Rev. Physiol.

    (1989)
  • B.P. Bean

    Pharmacology of calcium channels in cardiac muscle, vascular muscle, and neurons

    Am. J. Hypertens.

    (1991)
  • B.P. Bean et al.

    Nonlinear charge movement in mammalian cardiac ventricular cells. Components from Na and Ca channel gating

    J. Gen. Physiol.

    (1989)
  • R.E. Fisher et al.

    Properties and distribution of single voltage-gated calcium channels in adult hippocampal neurons

    J. Neurophysiol.

    (1990)
  • A.P. Fox et al.

    Singlechannel recordings of three types of calcium channels in chick sensory neurones

    J. Physiol. (Lond.)

    (1987)
  • A.P. Fox et al.

    Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones

    J. Physiol. (Lond.)

    (1987)
  • O.P. Hamill et al.

    Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

    Pflügers Arch.

    (1981)
  • P. Hess

    Calcium channels in vertebrate cells

    Annu. Rev. Neurosci.

    (1990)
  • D. Hillman et al.

    Localization of P-type calcium channels in the central nervous system

  • Cited by (676)

    • Toxicology of the Neuromuscular Junction

      2018, Comprehensive Toxicology: Third Edition
    • Ion Channels

      2018, Comprehensive Toxicology: Third Edition
    • The Tottering Mouse

      2023, Essentials of Cerebellum and Cerebellar Disorders: A Primer For Graduate Students, Second Edition
    View all citing articles on Scopus
    View full text