Elsevier

Neuroscience Letters

Volume 51, Issue 3, 26 October 1984, Pages 325-330
Neuroscience Letters

Antinociceptive effects in mice after intrathecal injection of 5′-N-ethylcarboxamide adenosine

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Abstract

When injected intrathecally in mice in a volume of 5 μl, adenosine had no effect on tail-flick or hot-plate reaction latencies at dosages up to 1 mM concentration. There were no other behavioral effects observed either. Injecting 1 mM of the adenosine receptor agonist, 5′-N-ethylcarboxamide adenosine (NECA) caused both motor paralysis of the hind-legs with a duration of approximately 4 h and simultaneous antinociception. A slight weakness of the hindlegs, but a profound antinociceptive effect, was observed after the 100 μM dose only. After 10 μM, there was no effect on motor behavior but still a prolongation of the tail-flick and hot-plate reaction latencies. Pretreatment with the adenosine receptor antagonist theophylline attenuated the antinociceptive effect of NECA. Activation of spinal adenosine receptors thus appears to selectively elicit analgesia.

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