Basal metallothionein in tumors: Widespread presence of apoprotein

https://doi.org/10.1016/0162-0134(94)80023-5Get rights and content

Abstract

A survey has been conducted of solid and ascites tumors from mice and solid tumors in rats for the presence of metallothionein or metallothionein-like protein. In most tumors, a positive identification was made on the basis of Sephadex G-75 and HPLC-DEAE chromatography followed by competitive radioimmunoassay for metallothionein. Apometallothionein was revealed in a number of tumors for the first time by comparing the Sephadex G-75 chromatographic profiles of Zn in native cytosol and Cd in cytosol incubated briefly with CdCl2 to saturate free binding sites on the protein before Sephadex G-75 chromatography. In two cases unsaturation of metallothionein was correlated with a lack of zinc in the ascites fluid which supplies the tumor with zinc.

References (50)

  • A. Bakka et al.

    Toxicol. Appl. Pharmacol.

    (1981)
  • A.J. Kraker et al.

    Biochem. Biophys. Res. Commun.

    (1985)
  • J. Zeng et al.

    FEBS Lett.

    (1991)
  • S.L. Feldman et al.

    Biochem. Biophys. Acta

    (1978)
  • O.H. Lowry

    J. Biol. Chem.

    (1951)
  • H.S. Reinhold

    Europ. J. Cancer

    (1966)
  • T. Hanke et al.

    FEBS Lett.

    (1988)
  • S.A. Metcalfe et al.

    Cancer Lett.

    (1986)
  • S. Onosaka et al.

    Toxicology

    (1986)
  • H.A. Hidalgo et al.

    Toxicol. Appl. Pharmacol.

    (1978)
  • T.P. Mulder et al.

    Fr. Rad. Biol. Med.

    (1990)
  • R.R. Bahnson et al.

    J. Urology

    (1991)
  • J.V. Wade et al.

    Life Sci.

    (1986)
  • B. Krauter et al.

    Biochem. Biophys. Acta

    (1989)
  • K.L. Bazzell et al.

    Toxicol. Appl. Pharmacol.

    (1979)
  • K.W. Mayo et al.

    J. Biol. Chem.

    (1981)
  • D. Hamer

    Ann. Rev. Biochem.

    (1986)
  • M.D. Enger et al.

    J. Toxicol. Environ. Health

    (1981)
  • P.A. Andrews et al.

    Cancer Chemother. Pharmacol.

    (1987)
  • L. Endresen et al.

    Cancer Res.

    (1983)
  • A. Glennas et al.

    Scand. J. Rhumatology

    (1985)
  • S.L. Kelley et al.

    Science

    (1988)
  • A.J. Kraker et al.

    Cancer Res.

    (1988)
  • S.K. Krezoski et al.

    Biochem. J.

    (1988)
  • J.D. Otvos et al.

    Comm. Inorg. Chem.

    (1990)
  • Cited by (0)

    1

    Department of Biology, Syracuse University, Syracuse, New York. Presently, Visiting Associate, California Institute of Technology, Pasadena, California.

    2

    Parke-Davis Pharmaceutical Research, Division of Warner Lambert Co., Ann Arbor, Michigan.

    View full text