Elsevier

Methods in Enzymology

Volume 206, 1991, Pages 577-587
Methods in Enzymology

[56] Isolation of P450 enzymes from human liver

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This chapter discusses the isolation of P450 enzymes from human liver. Considerable progress has been made in purifying and characterizing P450 enzymes from experimental animals. Although the information gathered has provided a foundation for the study of human P450 enzymes, there are limitations when extrapolating from experimental animals to humans. First, orthologous animal and human P450s differ in their catalytic specificity. Second, sexual bimorphism among P450 enzymes occurs in animals but probably not in humans. Experimental animals also give a biased view of differences among the human population, in that animals are not completely outbred and they consume defined diets. The genetically determined variations that exist in the structure and function of human P450 enzymes are rarely encountered in experimental animals.

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