Elsevier

Thrombosis Research

Volume 44, Issue 1, 1 October 1986, Pages 75-84
Thrombosis Research

Paper
Inhibition of the effects of thrombin on guinea pig platelets by the diacylglycerol lipase inhibitor rhc 80267

https://doi.org/10.1016/0049-3848(86)90182-9Get rights and content

Abstract

Phospholipase C (PLC) and diacylglycerol lipase (DGL) activities were found in guinea pig platelet microsome preparations. No phospholipase A2 (PLA2) activity was detected. RHC 80267 (1,6-di (0-(carbamoyl) cyclohexanone oxime)hexane) inhibited DGL activity (IC50 = 4 μM) from guinea pig platelet microsomes but had no effect on PLC. RHC 80267 inhibited platelet aggregation (IC50 = 11 μM), release of arachidonic acid (AA), its metabolites, and ATP (IC50 = 4.5 μM) when guinea pig platelets were challenged with a low concentration of thrombin. We propose that PLC-DGL is an important enzymatic pathway for the release of AA in guinea pig platelets.

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