Cannabinod receptor and their ligandArachidonoyl ethanolamide-[1,2-14C] as a substrate for anandamide amidase
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Chronic, noninvasive glucocorticoid administration suppresses limbic endocannabinoid signaling in mice
2012, NeuroscienceCitation Excerpt :values were determined by nonlinear curve fitting of specific binding data to the single site binding equation using GraphPad Prism (San Diego, CA, USA). FAAH activity was measured as the conversion of AEA labeled with [3H] in the ethanolamine portion of the molecule ([3H]AEA; Omeir et al., 1995) to [3H]ethanolamine as reported previously (Hillard et al., 1995). Membranes were incubated in a final volume of 0.5 ml of TME buffer (50 mM Tris–HCl, 3.0 mM MgCl2, and 1.0 mM EDTA, pH 7.4) containing 1.0 mg/ml fatty acid-free bovine serum albumin and 0.2 nM [3H]AEA.
Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2
2010, Journal of Biological ChemistryCitation Excerpt :This suggests that in vitro activities, employing high substrate concentrations, do not reveal the true catabolic capacities of NAE-inactivating enzymes in cells. The elevated activity of FAAH-2 in intact cells is supported by its low Km values for AEA and PEA, which are severalfold lower than those reported for FAAH (25, 26). This suggests higher enzymatic activity at physiological substrate concentrations (i.e. ≤1 μm), such as those used in our intact cell system.
Microglia produce and hydrolyze palmitoylethanolamide
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