[3H]CGP 39653: a new N-methyl-D-aspartate antagonist radioligand with low nanomolar affinity in rat brain
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2017, European Journal of Medicinal ChemistryCitation Excerpt :Rat brain membrane preparations used in native receptor binding experiments were prepared according to the method described by Ransom and Stec [37]. Affinity for AMPA [38] KA [39] and NMDA [40] receptor sites was determined using 5 nM [3H]AMPA, 5 nM [3H]KA, and 2 nM [3H]CGP 39653 (all from Perkin Elmer, Waltham, MA) with some modifications as previously described [41]. Recombinant rat AMPA receptor binding.
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Ciba-Geigy Pharmaceutical Research Division, CH-4002 Basel, Switzerland.
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