The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts
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Intracellular ascorbate enhances hypoxia-inducible factor (HIF)-hydroxylase activity and preferentially suppresses the HIF-1 transcriptional response
2014, Free Radical Biology and MedicineCitation Excerpt :Ascorbate was relatively stable in medium under our experimental conditions, with a half-life of ~5–6 h (Supplementary Fig. 1). This is somewhat longer than reported by others in medium without cells or serum [25], but is similar to the findings of others who also monitored ascorbate in culture medium with serum and cells [26,27] and most likely reflects the presence of protective factors (serum iron chelators) in these conditions. Intracellular uptake peaked at 8 h and remained stable for up to 48 h.
Ascorbic Acid and Cancer: Animal and Cell Culture Data
2012, Natural Antioxidants in Human Health and DiseaseIGF-I and vitamin C promote myogenic differentiation of mouse and human skeletal muscle cells at low temperatures
2011, Experimental Cell ResearchCitation Excerpt :VC plays various essential roles in vivo, which includes serving as a cofactor for the hydroxylation of proline and lysine during collagen synthesis. Although L-ascorbic acid is unstable and rapidly degraded under the normal culture conditions [13], a phosphate derivative of L-ascorbic acid is considerably stable, with 85% still remaining after a 1-week incubation at 37 °C [14]. L-ascorbic acid phosphate was reported to increase myogenin expression in muscle cells and promote their myogenic differentiation by accelerating collagen synthesis at 37 °C [15,16].
A new dermal equivalent: The use of dermal fibroblast culture alone without exogenous materials
2006, Journal of Dermatological Science