A unified method for the assay of uridine diphosphoglucuronyl-transferase activities toward various aglycones using uridine diphospho[U-14C]glucuronic acid
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The cucurbitacins E, D and I: Investigation of their cytotoxicity toward human chondrosarcoma SW 1353 cell line and their biotransformation in man liver
2013, Toxicology LettersCitation Excerpt :A same protocol described above was performed on calibration curves. Glucuronidation of cucurbitacins I, D and E by human and rat liver microsomes or by the different human recombinant isoforms of UDP-glucuronosyltransferases was performed by a modified method of Bansal and Gessner (1980). The activity of the recombinant enzymes was previously checked by the same method with marker substrates: octylgallate for UGT1A1, 4-aminobiphenyl for UGT1A4, 1-naphthol for UGT1A6, UGT1A7, UGT1A8 and UGT1A9, β-estradiol for UGT1A10, hyodeoxycholic acid for UGT2B4, RS-ketoprofen for UGT2B7, eugenol for UGT2B15 and UGT2B17.
Metabolism of parabens (4-hydroxybenzoic acid esters) by hepatic esterases and UDP-glucuronosyltransferases in man
2010, Drug Metabolism and PharmacokineticsInduction of antioxidant and phase 2 drug-metabolizing enzymes by falcarindiol isolated from Notopterygium incisum extract, which activates the Nrf2/ARE pathway, leads to cytoprotection against oxidative and electrophilic stress
2009, Archives of Biochemistry and BiophysicsCitation Excerpt :Catalase activity was determined using hydroperoxide as a substrate [23]. UGT activity toward 4-methylumbelliferone (4-MU) was measured by TLC-radioluminography according to the procedures described previously [24]. The reaction mixture (50 μl) contained 50 mM Tris–HCl (pH 7.5), 10 mM MgCl2, 0.5 mM 4-MU, 2 mM [14C]UDPGA (9.25 kBq), and 0.8 μg/ml of microsomes prepared from cell lysates by differential centrifugation at 105,000g for 60 min.