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Temperature sensitivity of Ca currents in chick sensory neurones

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Abstract

We have investigated the effects of temperature on the Ca currents of chick sensory neurones. Raising the temperature from 17 to 37°C, caused low-threshold (LVA, T) and high-threshold (HVA, L and N) Ca currents to show a marked amplitude increase and a drastic acceleration of their activation-inactivation gatings. Compared to HVA channels, the LVA type showed a weaker temperature sensitivity. Its averageQ 10 values were closer to those of other voltage-operated ion channels: 1.7 (permeability), 1.9 (activation) and 2.2 (inactivation). Alternatively, the activation kinetics and peak permeability of HVA Ca channels showed maximalQ 10 values of about 5 and 2.8, respectively. HVA channel deactivation was less sensitive to temperature (Q 10 1.8). Inactivation of these channels was slow and monoexponential between 17 and 22°C, but faster and double exponential above 30°C, uncovering a fast temperature-sensitive decaying phase. The size and rate of decay of this component decreased with increasing membrane depolarizations and persisted at holding potentials positive to −80 mV, suggesting the involvement of temperature-sensitive Ca-mediated processes in the mechanism of HVA channel inactivation. Our data are consistent with the view that heating from 17 to 37°C causes both an increased probability of Ca channels to open and a drastic acceleration of their activation-inactivation kinetics.

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References

  • Bean B (1989) Classes of calcium channels in vertebrate cells. Annu Rev Physiol 51:367–384

    Article  PubMed  CAS  Google Scholar 

  • Brown AM, Tsuda Y, Wilson DL (1983) A description of activation and conduction in calcium channels based on tail and turn-on current measurements in the snail. J Physiol (Lond) 344:549–583

    CAS  Google Scholar 

  • Brown AM, Lux HD, Wilson DL (1984) Activation and inactivation of single calcium channels in snail neurons. J Gen Physiol 83:751–769

    Article  PubMed  CAS  Google Scholar 

  • Byerly L, Chase PB, Stimers JR (1984) Calcium current activation kinetics in neurones of the snailLymnaea Stagnalis. J Physiol (Lond) 348:187–207

    CAS  Google Scholar 

  • Carbone E, Lux HD (1984a) A low voltage-activated calcium conductance in embryonic chick sensory neurons. Biophys J 46:413–418

    Article  PubMed  CAS  Google Scholar 

  • Carbone E, Lux HD (1984b) A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurons. Nature 310:501–502

    Article  PubMed  CAS  Google Scholar 

  • Carbone E, Lux HD (1987a) Kinetics and selectivity of a lowvoltage-activated calcium current in chick and rat sensory neurones. J Physiol (Lond) 386:547–570

    CAS  Google Scholar 

  • Carbone E, Lux HD (1987b) Single low-voltage-activated calcium channels in chick and rat sensory neurones. J Physiol (Lond) 386:571–601

    CAS  Google Scholar 

  • Cavaliè A, McDonald TF, Pelzer D, Trautwein W (1985) Temperature-induced transitory and steady-state changes in the calcium current of guinea pig ventricular myocytes. Pflügers Arch 405:294–296

    Article  PubMed  Google Scholar 

  • Chiu SY, Mrose HE, Ritchie JM (1979) Anomalous temperature dependence of the sodium conductance in rabbit nerve compared with frog nerve. Nature 279:327–328

    Article  PubMed  CAS  Google Scholar 

  • Colatsky TJ (1980) Voltage-clamp measurements of sodium channel properties in rabbit cardiac Purkinje fibres. J Physiol (Lond) 305:215–234

    CAS  Google Scholar 

  • Eckert R, Chad JE (1984) Inactivation of Ca2+ channels. Prog Biophys Mol Biol 44:215–267

    Article  PubMed  CAS  Google Scholar 

  • Fenwick EM, Marty A, Neher E (1982) Sodium and calcium channels in bovine chromaffine cells. J Physiol (Lond) 331:599–635

    CAS  Google Scholar 

  • Frankenhaeuser B, Moore LE (1963) The effect of temperature on the sodium and potassium permeability changes in myelinated nerve fibres ofXenopus laevis. J Physiol (Lond) 169:431–437

    CAS  Google Scholar 

  • Fukushima Y, Hagiwara S (1985) Currents carried by monovalent cations through calcium channels in mouse neoplastic B lymphocytea. J Physiol (Lond) 358:255–284

    CAS  Google Scholar 

  • Galietta LJV, Galdzicki Z, Nobile M (1988) Low Ca2+-sensitive maxi-K+ channels in human cultured fibroblasts. Pflügers Arch 413:99–101

    Article  PubMed  CAS  Google Scholar 

  • Gutnick MJ, Lux HD, Swandulla D, Zucker H (1989) Voltage-dependent and calcium-dependent inactivation of calcium channel current in identified snail neurones. J Physiol (Lond) 412:197–220

    CAS  Google Scholar 

  • Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100

    Article  PubMed  CAS  Google Scholar 

  • Kimura JE, Meves H (1979) The effect of temperature on the asymmetrical charge movement in squid giant axons. J Physiol (Lond) 289:479–500

    CAS  Google Scholar 

  • Kniffki KD, Siemen D, Vogel W (1981) Development of sodium permeability inactivation in nodal membranes. J Physiol (Lond) 313:37–48

    CAS  Google Scholar 

  • Kostyuk PG, Krishtal OA, Pidoplichko VI (1981) Calcium inward currents and related charge movements in the membrane of snail neurones. J Physiol (Lond) 310:403–421

    CAS  Google Scholar 

  • Lux HD, Brown AM (1984) Patch and whole cell calcium currents recorded simultaneously in snail neurons. J Gen Physiol 85:727–750

    Article  Google Scholar 

  • Narahashi T, Tsunoo A, Yoshii M (1987) Characterization of two types of calcium channels in mouse neuroblastoma cells. J Physiol (Lond) 383:231–249

    CAS  Google Scholar 

  • Nowycky MC, Fox AP, Tsien RW (1985) Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature 316:440–443

    Article  PubMed  CAS  Google Scholar 

  • Schwarz JR (1986) The effect of temperature on Na currents in rat myelinated nerve fibres. Pflügers Arch 406:397–404

    Article  PubMed  CAS  Google Scholar 

  • Schwarz W (1979) Temperature experiments on nerve and muscle membranes of frogs. Indications for a phase transition. Pflugers Arch 382:27–34

    Article  PubMed  CAS  Google Scholar 

  • Swandulla D, Armstrong CM (1988) Fast deactivating calcium channels in chick sensory neurons. J Gen Physiol 92:197–218

    Article  PubMed  CAS  Google Scholar 

  • Taylor WR (1988) Two-suction-electrode voltage-clamp analysis of the sustained calcium current in cat sensory neurones. J Physiol (Lond) 407:405–432

    CAS  Google Scholar 

  • Tsien RW, Lipscombe D, Madison DV, Bley KR, Fox AP (1988) Multiple types of neuronal calcium channels and their selective modulation. Trends Neurosci 11:431–438

    Article  PubMed  CAS  Google Scholar 

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Nobile, M., Carbone, E., Lux, H.D. et al. Temperature sensitivity of Ca currents in chick sensory neurones. Pflugers Arch. 415, 658–663 (1990). https://doi.org/10.1007/BF02584002

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  • DOI: https://doi.org/10.1007/BF02584002

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