Abstract
Kindling epileptogenesis was induced by periodic electrical stimulation of the Schaffer collateral/commissural pathway in the CA1 area of the rat hippocampus. The progressive nature of hippocampal kindling is demonstrated by a detailed description of the behavioral signs and the progressive increase of the after-discharge duration in the course of kindling acquisition. Furthermore, the evolution of the alterations in the paired-pulse local evoked field potentials and the modifications of the GABAA receptor binding and of the expression of mRNAs encoding for the subunits of the GABAA and glutamate receptors are considered. Evidence is presented that during kindling opposite changes occur in the CA1 and the fascia dentata in terms of the balance between excitation and inhibition due to contrasting changes in GABA-mediated inhibitory function.
Sommario
Mediante stimolazione elettrica periodica della via commissurale/collaterale di Schaffer nell'area CA1 dell'ippocampo di ratto è stata indotta una epilessia legata al meccanismo del “kindling”. La natura progressiva del fenomeno è dimostrata da una dettagliata descrizione di segni comportamentali e del progressivo incremento della durata della scarica durante il “kindling”. In aggiunta sono state considerate: l'evoluzione delle alterazioni dei potenziali di campo evocati da coppie di pulsi, le modificazioni del legame recettoriale per i GABAA, l'espressione dell'mRNAs che codifica per le subunità recettoriali del GABAA e Glutamato. Vengono qui presentate le evidenze secondo cui durante il kindling avvengono in CA1 e nella fascia dentata modificazioni opposte in termini di bilancio tra eccitazione ed inibizione dovuti a cambiamenti contrastanti della funzione inibitoria GABA-mediata.
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This work was supported by grants from the Committee on Epilepsy of the National Epilepsy Fund, Utrecht, and from the Organization of Scientific Research of the Netherlands, Division of Medical Science (NWO), The Hague
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Lopes da Silva, F.H., Kamphuis, W., Titulaer, M. et al. An experimental model of progressive epilepsy: the development of kindling of the hippocampus of the rat. Ital J Neuro Sci 16, 45–57 (1995). https://doi.org/10.1007/BF02229074
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DOI: https://doi.org/10.1007/BF02229074