Summary
Transcardial perfusion or intraperitoneal injections with sodium selenite result in the creation of selenium bonds that can be visualized by physical development. The present paper describes how these catalytic bonds are made visible in the tissues by surrounding them with shells of metallic silver. Based on experiments with chelating agents, the possibility that selenium-metal bonds are the catalysts is discussed. In the brain, the selenium pattern is delicate and highly laminated, the grains of silver being orderly arranged corresponding with the neuropil morphology. The precipitate is most densely packed in cortical regions. The difference in staining intensity seen in different regions of the CNS reflects the density of selenium reactive terminals. The visualized selenium bonds are predominantly located within boutons, and examination in the electron microscope reveals accumulation in the presynaptic regions. In a few places precipitates can also be found in axons, but have not been observed in perikarya or dendrites. The only non-neuronal locations of selenium were sparsely scattered, astrocyte-like neuroglia, predominantly found in the cerebellum and the hypothalamus; infrequently a few blood vessels were also stained. Sections from kidney and liver are presented as examples of localizations outside the CNS of exogenous selenium.
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References
Aaseth J, Olsen A, Halse J, Hovig T (1981) Argyria-tissue deposition of silver as selenide. Scand J Clin Lab Invest 41:247–251
Bayard JL (1969) Trimethyl selenide. A urinary metabolite of selenite. Arch Biochem Biophys 130:556–560
Burk RF (1973) 75Se-binding by rat plasma proteins after injection of 75SeO −23 . US Army Med Res Nutr Lab Rep 334
Cerwenka EA Jr, Cooper WC (1961) Toxicology of tellurium and selenium and their compounds. Arch Ind Health 3:189–200
Cummins LM, Martin JL (1967) Are selenocystine and selenomethionine synthesized in vivo from sodium selenite in mammals. Biochemistry 6:3163–6168
Danscher G (1981a) Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy. Histochemistry 71:1–16
Danscher G (1981b) Light and electron microscopic localization of silver in biological tissue. Histochemistry 71:177–186
Danscher G, Haug F-MŠ, Fredens K (1973) Effect of diethyldithiocarbamate (DEDTC) on sulphide silver stained boutons. Reversible blocking of Timm's sulphide silver stain for “heavy” metals in DEDTC treated rats (light microscopy). Exp Brain Res 16:521–532
Diplock AT (1976) Metabolic aspects of selenium action and toxicity. CRC Crit Rev Toxicol 4:271–329
Ganther HE, Corcoran C (1969) Selenotrisulphides. II. Cross-linking of reduced pancreatic ribonuclease with selenium. Biochemistry 7:2557–2563
Jenkins KJ (1968) Evidence for the absence of selenocystine and selenomethionine in the serum proteins of chicks administered selenite. Can J Biochem 46:1417–1425
Levander OA, Morris VC, Higgs DJ (1973) Acceleration of thiolinduced swelling of rat liver mitochondria by selenium. Biochemistry 12:4586–4590
Meyer RJ (1924) Gmelins Handbuch der anorganischen Chemie. Verlag Chemie, Weinheim/Bergstrasse, pp 246–247
Palmer IS, Fischer DD, Halverson AW, Olson OE (1969) Identification of a major selenium excretory product in rat urine. Biochim Biophys Acta 177:336–342
Stadtman TC (1974) Selenium biochemistry. Science 183:915–922
Strømme JH (1965) Metabolism of disulfiram and diethyldithiocarbamate in rats with demonstration of an in vivo ethanol-induced inhibition of the glucuronic acid conjugation of the thiol. Biochem Pharmacol 14:393–410
Timm F (1958) Zur Histochemie der Schwermetalle. Das Sulfid-Silber-Verfahren. Dtsch Z ges gerichtl Med 46:706–711
Venugopal B, Luckey TD (1974) Toxicology of non-radioactive heavy metals and their salts. Environ Qual Saf Suppl 2:4–73
Williams RJP (1978) A short note on selenium biochemistry. In: Williams RJP, Da Silva JRRF (eds) New Trends in Bio-inorganic Chemistry. Academic Press. New York, pp 253–260
Zeiger K (1938) Physikochemische Grundlagen der histologischen Methodik. Eill Forschungsber 48:55–105
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Danscher, G. Exogenous selenium in the brain. Histochemistry 76, 281–293 (1982). https://doi.org/10.1007/BF00543951
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DOI: https://doi.org/10.1007/BF00543951