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Activation of Gi1by Lysophosphatidic Acid Receptor without Ligand in the Baculovirus Expression System

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Abstract

Lysophosphatidic acid (LPA) receptor has been attracting many neuroscientists' concerns, since it was reported to have a potential role in the neurogenesis, which occurs in the ventricular zone of the developing and adult brain. In the present experiments using baculovirus expression system, the LPA receptor encoded by ventricular zone gene 1 (Edg-2/Vzg-1) was found to be functionally coupled to Gi1, Goa, and G11, but not to GS. The coexpression of LPA receptor markedly decreased the expression of G protein αi1or αoasubunit, while the basal [35S]GTPγS binding significantly increased in the Gi1-preparation. The Scatchard Plot analysis indicates that the expression of LPA-receptor (Edg-2/Vzg-1) showed stimulation of Gi1without agonist. These results suggest the Edg-2/Vzg-1 has an intrinsic acctivity on Gi1.

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    Parts of this study were supported by Grants-in-Aid from the Ministry of Education, Science, Culture, and Sports of Japan, and by a research grant from Ono Medical Research Foundation.

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    To whom correspondence and reprint requests should be addressed at Department of Molecular Pharmacology and Neuroscience, Nagasaki University School of Pharmaceutical Sciences, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Fax: +81-95-844-4248. E-mail:[email protected].

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