Regular Article
Dopamine D1 Receptor Mutagenesis: Role of Amino Acids in Agonist and Antagonist Binding

https://doi.org/10.1006/bbrc.1993.1319Get rights and content

Abstract

We investigated the role of amino acids, by site-directed mutagenesis, in five of the seven transmembrane regions of the human dopamine D1 receptor. The results demonstrate a role for an aspartic acid (Asp70) in transmembrane 2 mediating the sodium ion effect on receptor conformation. Amino acids residues in transmembrane 3 and 5 are important for optimum dopamine binding to the receptor. Mutant receptors involving amino acids in transmembrane 7 suggest that binding sites for agonists and antagonists have distinct binding determinants within the dopamine D1 receptor.

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