Regular ArticleMeasurement of Responses from Gi-, Gs-, or Gq-Coupled Receptors by a Multiple Response Element/cAMP Response Element-Directed Reporter Assay
References (24)
- et al.
Human AT1 receptor is a single copy gene: Characterization in a stable cell line
Mol. Cell. Biochem.
(1994) - et al.
Expression and characterization of cloned human bombesin receptors
Mol. Pharmacol.
(1995) - et al.
Melanin-concentrating hormone is the natural cognate ligand for the orphan G-protein coupled receptor SLC-1
Nature
(1999) - et al.
A colorimetric assay for measuring activation of Gs- and Gq-coupled signaling pathways
Anal. Biochem.
(1995) - et al.
Crosstalk during Ca2+-, cAMP, and glucocorticoid-induced gene expression in lymphocytes
Mol. Cell. Endocrinol.
(1997) - et al.
Structure of the gene for human beta 2-adrenergic receptor: Expression and promoter characterization
Proc. Natl. Acad. Sci. USA
(1987) - et al.
Characterization of the murine pancreatic receptor for gastrin releasing peptide and bombesin
Regul. Peptides
(1991) - et al.
The CGTCA sequence motif is essential for biological activity of the vasoactive intestinal peptide gene cAMP-regulated enhancer
Proc. Natl. Acad. Sci. USA
(1988) - et al.
Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133
Cell
(1989) - et al.
Functional testing of human dopamine D1 and D5 receptors expressed in stable cAMP-responsive luciferase reporter cell lines
J. Receptor Res.
(1993)
Method for identifying ligands that bind to cloned Gs- or Gi-coupled receptors
Mol. Cell Neurosci.
Cited by (65)
Novel pyrrolidinone derivative lacks claimed histamine H<inf>3</inf> receptor stimulation in receptor binding and functional studies
2020, European Journal of Medicinal ChemistryCitation Excerpt :GTPγ[35S] dependent binding activity was determined in vitro for histamine, imetit, (R)-α-methylhistamine and the compound of interest. Chinese hamster ovary cells (CHO-DUKX, ATCC No. CRL-9010) expressing stable human histamine H3 receptor cDNA (GenBank accession No. NM_007232) and stable multiple-responsive element/cAMP-responsive-element driving luciferase gene reporter were used [36]. The assay was performed with non-adherent cells in a 96 wells format using 20,000 cells per well in alphaMEM without serum in the presence of IBMX (500 μM).
Role of histamine H<inf>4</inf> receptor ligands in bleomycin-induced pulmonary fibrosis
2016, Pharmacological ResearchIntegrated approaches to understanding antipsychotic drug action at GPCRs
2014, Current Opinion in Cell BiologyCitation Excerpt :However, the discovery of several families of G proteins [14], the cloning of GPCRs and the observation that a GPCR can activate multiple G proteins [15] have in recent years caused an explosion in the development of assays that can reveal the regulation of multiple downstream signaling cascades. Several high-throughput screening (HTS) assays have been developed such as aequorin-based fluorescent assays [16] or cyclic adenosine monophosphate (cAMP) response element-directed reporter assays [17] to measure Gαq, Gαi, and Gαs signaling events. Additionally, fluorescence resonance energy transfer assays, such as a protein kinase A (PKA) cAMP sensor [18] and an EPAC-based sensor [19], have been developed to study Gαi and Gαs signaling downstream of several GPCRs.
Development of a novel DnaE intein-based assay for quantitative analysis of G-protein-coupled receptor internalization
2011, Analytical BiochemistryCitation Excerpt :Transfected cells were prepared for the assay 48 h after transfection. The cAMP accumulation was measured through an assay that used a pCRE-fLuc vector (Clontech) consisting of the firefly luciferase coding region under control of a minimal promoter containing cAMP response elements (CREs) [23]. The cells were seeded in a 48-well plate overnight before the beginning of the assay.
Serendipitous discovery of a new class of agonists for the melanocortin 1 and 4 receptors and a new class of cyclophanes
2011, Bioorganic and Medicinal Chemistry LettersVav1 couples the T cell receptor to cAMP response element activation via a PKC-dependent pathway
2010, Cellular Signalling