Regular ArticleMeasurement of Cytochrome P450 2E1 Activity in Rat Tracheobronchial Airways Using High-Performance Liquid Chromatography with Electrochemical Detection☆
References (17)
- et al.
Toxicol. Appl. Pharmacol.
(1996) - et al.
Biochem. Biophys. Res. Commun.
(1994) - et al.
Toxicol. Appl. Pharmacol.
(1995) - et al.
Anal. Biochem.
(1993) Anal. Biochem.
(1976)- et al.
DNA Cell Biol.
(1993) Drug Metab. Rev.
(1995)- et al.
Chem. Res. Toxicol.
(1991)
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Modulating effect of graphine oxide loaded hesperidin nanocomposite on the 1,2-dimethylhydrazine provoked colon carcinogenesis in rats via inhibiting the iNOS and COX-2 pathways
2020, Arabian Journal of ChemistryCitation Excerpt :Reduced glutathione (GSH) was resolute by Ellman method (1959) glutathione peroxidase (GPx) levels were scrutinized by the technique of Rotruck et al. (1973) and ultimately glutathione reductase (GR) measurement was done by Carlberg and Mannervik (1985). Cytochrome P450 was considered by the process of Omura and Sato (1964) and Cytochrome 4502E1 inference was done by the protocol of Watt et al. (1997)). GST was assessed by the means of Habig et al. (1974).
Orientin, a flavanoid, mitigates 1, 2 dimethylhydrazine-induced colorectal lesions in Wistar rats fed a high-fat diet
2018, Toxicology ReportsCitation Excerpt :Cytochrome P4502E1 (CYP2E1, EC 1.14.13. n7) was measured by the method of Watt et al. [34]. The activity of glutathione S-transferase (GST, EC 2.5.1.18) was determined by Habig et al. [35] method.
Asiatic acid abridges pre-neoplastic lesions, inflammation, cell proliferation and induces apoptosis in a rat model of colon carcinogenesis
2017, Chemico-Biological InteractionsCitation Excerpt :The rapid diminution of cytochrome b5 was characterized by an increase in absorbance at 427 nm. Cytochrome P4502E1 activity was measured by the method of [49]. The reaction mixture contained 100 μg microsomal protein, 40 mM p-nitrophenol and 0.1 M phosphate buffer.
Antihyperlipidemic and antiapoptotic potential of zingerone on alcohol induced hepatotoxicity in experimental rats
2017, Chemico-Biological InteractionsCitation Excerpt :0.25 M sucrose was added and centrifuged at 9000 g for 20 min, to obtain microsomal fractions, which were promptly assayed for phase I enzymes. Cytochrome P450 (EC 1.14.14.1) and cytochrome b5 were determined by the method of Omura and Sato [34], cytochrome P4502E1 (EC 1.14.14.1) by the method of Watt et al. [35], NADH-cytochrome P450 reductase (EC 1.6.2.2) by the method of Mihara and Sato [36] and NADPH-cytochrome b5 reductase (EC 1.6.2.4) by the method of Omura and Takesue [37]. DT-diaphorase (EC.1.6.9.92) activity was assayed by the method of Ernster et al. [38] and glutathione S-transferase (GST, EC.2.5.1.18) by the method of Habig et al. [39].
Biochemical and molecular mechanisms underlying the chemopreventive efficacy of rosmarinic acid in a rat colon cancer
2016, European Journal of Pharmacology
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E. WattermanE. Johnson, Eds.