Regular ArticleTransient Kinetics of Intracomplex Electron-Transfer in the Human Cytochrome b5 Reductase-Cytochrome b5 System: NAD+ Modulates Protein-Protein Binding and Electron Transfer
References (0)
Cited by (16)
Topography of human cytochrome b<inf>5</inf>/cytochrome b<inf>5</inf> reductase interacting domain and redox alterations upon complex formation
2018, Biochimica et Biophysica Acta - BioenergeticsCitation Excerpt :Therefore, results allowed us to titrate the fluorescence increment induced by the presence of Cb5 upon its concentration allowing to calculate a dissociation constant for the complex formation (Kd = 0.5 ± 0.1 μM) (Fig. 1). The calculated values are in range of reported values found for this complex formation by laser flash photolysis [42] and similar to that found for the complex formation between Cb5 and P450cam [43]. These results allowed us to determine the stoichiometry of the complex to be used in the remaining experiments.
Study of the individual cytochrome b<inf>5</inf>and cytochrome b <inf>5</inf> reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain
2010, Journal of Biological ChemistryCitation Excerpt :The efficiency of Cyb5A reduction by Cyb5R3 decreased 4-fold when the solution ionic strength was increased from 0.009 m (buffer i) to 0.108 m (buffer ii), as measured by kinetic studies performed at a single substrate concentration (Table 2). This is indicative of a strong electrostatic component to docking between the molecules, consistent with previous reports (60, 61). A similar decrease in catalytic efficiency was observed in analogous experiments performed with Ncb5or-b5 and Ncb5or-b5R (Table 2), suggesting that electrostatic interactions contribute to docking between this redox pair as well.
Phase I biotransformation reactions-NADH-cytochrome b5 reductase
2007, xPharm: The Comprehensive Pharmacology ReferenceRole of Thr<sup>66</sup> in porcine NADH-cytochrome b<inf>5</inf> reductase in catalysis and control of the rate-limiting step in electron transfer
2003, Journal of Biological ChemistryUsefulness of statistic experimental designs in enzymology: Example with recombinant hCYP3A4 and 1A2
1999, Analytical Biochemistry