TABLE 1

Incubation conditions for the 11 substrates investigated in human intestinal and liver microsomes


Substrate

HLM

HIM

Substrate Concentration Range
Protein
Time
Protein
Time
mg/ml min mg/ml min μM
Midazolam 0.25 5 0.5 10 1-200
Triazolam 0.25 15 1 30 1-1000
Alprazolam 0.25 20 1.5 30 1-1000
Diazepam 0.25 15 1.5 30 2.5-500
Flunitrazepam 0.25 20 1 30 1-1000
Quinidine 0.5 15 1 30 1-400
Testosterone 0.5 15 1 20 1-500
Nifedipine 0.25 10 1 30 1-200
S-mephenytoin 1 60 1.5 60 1-1000
Tolbutamide 0.5 60 1.5 90 1-1000
Paclitaxel 0.5 10 1 20 1-200
Bufuralol
0.5
15
1
20
1-1000