TABLE 1

Inhibition of specific [³H]LBT-999 binding in rat striatal membranes

A mixture of 10 pmol of [³H]LBT-999 and 10 nM unlabeled LBT-999 was incubated with 30 μg of protein in Tris-HCl buffer, pH 7.4, for 90 min at 22°C in the presence of different drugs at concentrations of 10^–11 to 10^–5 M. Total binding was determined in the absence of drugs and nonspecific binding in the presence of 1 μM PE2I. IC₅₀ values were determined graphically. Values are means ± S.D. of three independent experiments each performed in triplicate.