Stimulatory effect of vNGF on migration of HAECs is not blocked by SU-5416
The OM assay was performed with 20,000 cells/ring. After 1 h, the ring was removed, the cells were gently washed with PBS, and the growth factors in EM were added in the presence or absence of drugs: 0.01% DMSO, 10 ng/ml VEGF, 50 ng/ml vNGF, and 0.25 μM SU-5416. After 3 days, the experiment was stopped, and the cell monolayers were photographed using a 20× magnification 2× objective. The migration was measured and analyzed according to Materials and Methods.
Growth Factor | Migration | ||
|---|---|---|---|
| DMSO | SU-5416 | ||
| % over control | |||
| Control | 100 | 106 ± 9 | |
| rhVEGF | 121 ± 6 | 99 ± 5* | |
| vNGF | 117 ± 7 | 118 ± 5 | |
↵* p < 0.01; statistical significance was calculated by either Student's t test comparing each experimental group to the respective control DMSO value or by one-way ANOVA (with post-Bonferroni's test) (n = 60).