Oligonucleotide primers and PCR conditions
Primer | Primer Sequences | Comments |
|---|---|---|
| UGT1A5 S | 5′-TGGCAATTATGAACAATATGTCT-3′ | Annealing temp. 56.1°C |
| UGT1A5 AS | 5′- GATGCATGGCTGACAAGAT-3′ | 30 cycles, 1.5 mM MgCl2 |
| GAPDH S | 5′-ACCCACTCCTCCACCTTTG-3′ | Annealing temp. 57.6°C |
| GAPDH AS | 5′-CTCTTGTGCTCTTGCTGGG-3′ | 34 cycles, 1.0 mM MgCl2 |
| UGT1A 3′-Spe1 | 5′-AGCCATACTAGTTCAATGGGTCTTGGATTTGT-3′ | See Materials and Methods |
| 1A5cDNAUTR | 5′-TTAAGACGAAGGAAACAATTC-3′ | |
| 1A5cDNAATG | 5′-AGCCATCTCGAGATGGCCACAGGACTCCAGGTTCCCCTGCCGCA-3′ |
|