TABLE 2

Summary of association experiment results using hSERT [125I]RTI-55 association time courses were conducted and analyzed as described under Materials and Methods. The indatraline and TB-1-099 experiments were done separately, requiring separate control conditions. The apparent kinetic Kd = Koff/Kon and the actual Kd were determined by binding surfaces conducted at the same time and using the same membranes as the association experiments.


Condition

Apparent

Actual Kd
Kon
Koff
Kinetic Kd
mol-1 min-1 min-1 nM
Control 0.032 ± 0.0037 0.015 ± 0.002 0.46 0.82 ± 0.03
TB-1-099 (1.5 μM) 0.016 ± 0.002 0.0168 ± 0.0026 1.02
TB-1-099 (15 μM) 0.009 ± 0.002 0.020 ± 0.006 2.20
Control 0.024 ± 0.002 0.015 ± 0.01 0.65 1.2 ± 0.1
Indatraline (2 nM) 0.0087 ± 0.0001 0.014 ± 0.002 1.61
Indatraline (10 nM)
0.0024 ± 0.0004
0.014 ± 0.004
5.83