Competition binding data showing pKi values for each tested relaxin family peptide at RXFP1, RXFP2 (100 pM and 1 nM labeled-H2 relaxin), RXFP1/2, and RXFP2/1
Values are expressed as means ± the standard error of the mean of four experiments.
H2 Relaxin | Porcine Relaxin | Rhesus Monkey Relaxin | Rat Relaxin | INSL3 | ||
|---|---|---|---|---|---|---|
| RXFP1 | 100 pM | 9.35 ± 0.04 | 8.56 ± 0.12 | 9.39 ± 0.02 | 7.27 ± 0.18 | N.A. |
| RXFP2 | 100 pM | 8.30 ± 0.15*** | 7.87 ± 0.60 | 7.81 ± 0.15*** | N.A.*** | 9.68 ± 0.13* |
| 1 nM | 8.34 ± 0.11 | 8.47 ± 0.44 | 7.71 ± 0.40 | N.A. | 9.19 ± 0.16†† | |
| RXFP1/2 | 100 pM | 9.09 ± 0.09** | 8.54 ± 0.17 | 9.09 ± 0.02*** | 7.67 ± 0.11* | 8.57 ± 0.57** |
| RXFP2/1 | 100 pM | 8.96 ± 0.05††† | 8.89 ± 0.24† | 7.93 ± 0.17 | N.A. | 9.91 ± 0.04† |
N.A., no affinity.
↵* p < 0.05, ** p < 0.01, and *** p < 0.001 versus related RXFP1 value (unpaired t test); †p < 0.05, ††p < 0.01, and †††p < 0.001 versus related RXFP2 value (unpaired t test).