Response to application of theophylline (theo, 30 mg/kg b.wt. i.v.) in A1 receptor wild-type (+/+, n = 5) and knockout (-/-, n = 5) mice anesthetized with α-chloralose/ketamine Clearance experiments were performed in two subsequent periods (P1 and P2, 30 min each). Theophylline was applied as a bolus after P1 and P2 was started 5 min thereafter. Data are means ± S.E.M.
+/+ Basal P1 | +/+ Theo P2 | -/- Basal P1 | -/- Theo P2 | |
|---|---|---|---|---|
| MAP (mm Hg) | 97 ± 9 | 107 ± 7* | 94 ± 12 | 114 ± 11* |
| HR (1/min) | 477 ± 30 | 609 ± 35* | 459 ± 36 | 597 ± 40* |
| Hct (%) | 45 ± 2 | 44 ± 2 | 46 ± 1 | 45 ± 2 |
| [Na+] plasma (mmol/l) | 149 ± 1 | 151 ± 2 | 148 ± 2 | 150 ± 2* |
| GFR (μl/min) | 207 ± 20 | 160 ± 21 | 191 ± 31 | 156 ± 33 |
| UV (μl/min) | 2.4 ± 0.3 | 4.6 ± 0.8* | 1.6 ± 0.2 | 1.9 ± 0.2 |
| AE Na+ (nmol/min) | 77 ± 8 | 387 ± 102* | 61 ± 10 | 105 ± 32 |
| FE Na+ (%) | 0.3 ± 0.1 | 1.7 ± 0.5* | 0.3 ± 0.1 | 0.5 ± 0.1 |
MAP, mean arterial pressure; HR, heart rate; Hct, arterial hematocrit; UV, urinary flow rate; AE, absolute urinary excretion; FE, fractional urinary excretion.
↵* p < 0.05 versus P1 same genotype