Estimates of CCh potency and maximal response range for M2 mAChR-mediated increases in whole-cell extracellular acidification rate in CHO cells under varying pretreatment conditions
Values are mean ± S.E.M. Cells were treated as indicated at 37°C, followed by extensive washout prior to assay.
Pretreatment (24 h) | Log EC50 | ECAR (Percentage of Basal) | n |
|---|---|---|---|
| Vehicle | −5.81 ± 0.13 | 36 ± 4 | 17 |
| 100 μM CCh | −5.40 ± 0.26 | 18 ± 3*** | 15 |
| 20 nM atropine | −5.99 ± 0.12 | 60 ± 7** | 16 |
| 20 μM gallamine | −5.78 ± 0.13 | 55 ± 5* | 13 |
Log EC50, logarithm of CCh EC50 value; ECAR (percentage of basal), maximal ECAR response range; n, number of experiments.
***p < 0.001 compared with vehicle pretreatment, as determined by one-way ANOVA; **p < 0.01 compared with vehicle pretreatment, as determined by one-way ANOVA; *p < 0.05 compared with vehicle pretreatment, as determined by one-way ANOVA.