TABLE 1

Comparison of IC50 values of anticancer drugs in metastatic and parental cells

The concentration (IC50) of each anticancer drug, which reduced cell growth by 50% after 96-h treatment, was determined from growth inhibition plots. The drug resistance fold represents increased degree of drug resistance of metastatic cells (M) compared with each parental cells (P). Data are calculated as the mean ± S.D. of triplicate samples.


Drug

Cells (IC50)

Resistance Fold (IC50)M/(IC50)P
KM12 KM12L4A
VP-16 (nM) 20 ± 1.2 170 ± 11 8.5***
BLM (μg/ml) 0.66 ± 0.02 2.10 ± 0.03 3.2***
DOX (ng/ml) 6.4 ± 0.2 11.0 ± 1.2 1.7**
LNCaP LNCaP-LN3
VP-16 (nM) 140 ± 9 672 ± 21 4.8***
BLM (μg/ml) 1.0 ± 0.1 3.8 ± 0.2 3.8***
DOX (ng/ml) 3.0 ± 0.1 6.4 ± 0.3 2.1***
A375 A375SM
VCR (nM) 5.3 ± 0.2 20.0 ± 1.8 3.8***
VBL (nM) 4.9 ± 0.3 9.6 ± 0.8 2.0**
DOX (ng/ml)

6.8 ± 0.4
1.8**
  • ** P < 0.01; ***P < 0.001.