TABLE 1

Kinetic parameters of the uptake of compounds by transporter-expressing HEK293 cells

Data shown in Fig. 1 were used to determine these parameters calculated by nonlinear regression analysis. Each point represents the mean ± S.E. (n = 3).

Transporter	Substrate	K_m	V_max	V_max/K_m	P_dif
		μM	pmol/min/mg	μl/min/mg	μl/min/mg
OATP2	E-sul	0.458 ± 0.154	60.3 ± 8.8	132 ± 19
	CCK-8		Not significantly transported
	Pitavastatin	3.00 ± 0.39	230 ± 17	76.6 ± 5.6
	E₂17βG	8.29 ± 0.42	131 ± 5	15.8 ± 0.6
OATP8	E-sul		Not significantly transported
	CCK-8	3.82 ± 2.20	102 ± 60	26.7 ± 15.7	11.4 ± 2.6
	Pitavastatin	3.25 ± 0.44	100 ± 8	30.6 ± 2.3
	E₂17βG	24.6 ± 7.6	56.8 ± 16.1	2.31 ± 0.65

P_dif, nonsaturable uptake clearance (microliters per minute per milligram of protein).