TABLE 3

Apparent binding constants obtained for expressed for control and tTEPA-inactivated CYP2B1 in the reconstituted system Assay conditions were as described under Materials and Methods. With the exception of the testosterone experiment, the data shown represent the means and standard deviations from at least three experiments.


Incubation Condition

Ks (μM)
Benzphetamine
Testosterone
n-Octylamine
+ tTEPA – NADPH 40 ± 7 79 0.76 ± 0.02
+ tTEPA + NADPH
72 ± 10
109
0.68 ± 0.01