TABLE 1

Comparison of saturation binding of [3H]EB with membrane homogenates from six stably transfected cell lines and rat forebrain Values shown are the mean ± S.E.M. of at least three independent experiments. See Fig. 1 for description of data analyses and curve fittings.


Subtype

Kd

Bmax
nM fmol/mg protein
α2β2 0.021 ± 0.003 340 ± 140
α2β4 0.084 ± 0.016 350 ± 70
α3β2 0.034 ± 0.006 64 ± 10
α3β4 0.29 ± 0.04 9700 ± 1400
α4β2 0.046 ± 0.012 430 ± 110
α4β4 0.094 ± 0.017 3500 ± 1300
Rat forebrain
0.054 ± 0.006
86 ± 5