TABLE 1

Purification of recombinant CES1A1 and CES2 Carboxylesterase activity was measured with 4-methylumbelliferyl acetate as the substrate. Purification data were from a 2-l preparation of Sf9 cells at the density of 2 × 106 cells/ml.

Enzyme
Total Activity
Total Protein
Specific Activity
μmol/min mg μmol/min/mg
CES1A1
    Whole cell lysate 155 400 0.4
    Concanavalin A 59 17 3.5
    Preparative PAGE 35 5 7
CES2
    Whole cell lysate 1360 440 3.1
    Concanavalin A 960 17 56
    Preparative PAGE
470
3.8
124