Oligonucleotide primers used for real-time PCR The nucleotide sequences of the respective up- and downstream primers are indicated.
Gene | Accession No. | 5′-Primer | 3′-Primer |
|---|---|---|---|
| β-Actin | NM_031144 | 5′-CCCCATTGAACACGGCATT | 5′-CATCTTTTCACGGTTGGCCTTA |
| GST-A3 | AF111160 | 5′-AGTCCTTCACTACTTCGATGGCAG | 5′-CACTTGCTGGAACATCAAACTCC |
| Aox1 | NM_019363 | 5′-GACCATGGAAGCTCACTAGTCCTCT | 5′-GGAACATAGGATCCAGGTTCATCTC |
| MT | M11794 | 5′-TGCAAGAAAAGCTGCTGTTCC | 5′-GTCCGAAGCCTCTTTGCAGAT |
| COXII | M64496 | 5′-CCAACCAATGACCTAAAACCAGG | 5′-GATTGCGTCGGTTTTTAACCCT |
| UGT | M13506 | 5′-CAGCAAAACTTTAGGAAGGCCC | 5′-GGTTTAGCAGGTTTGCAGTGGA |
| Cyclin D1 | D14014 | 5′-TACCCTGACACCAATCTCCTCAAC | 5′-CCTCTGCACGCACTTGAAGTAAG |
| Afar | NM_013215 | 5′-TCTCAACTGTACATGGACCGCTACT | 5′-CCCTTGAGCTGTGAGTGATGGTA |
Afar, aflatoxin B1 aldehyde reductase; Aox1, aldehyde oxidase; COXII, cytochrome c oxidase subunits II; MT, metallothionein-2 and metallothionein-1 genes; UGT, UDP-glucuronosyltransferase-phenobarbital (pb)-inducible form.