N-De-ethylating activities by rat recombinant CYP3A forms. The enzyme activity was measured using 50 μg of microsomal protein of COS-1 cells described in detail under Materials and Methods. Activities represented are the mean and the standard deviation of three different experiments by nmol/min/nmol P450.
P450 | Substrate | ||
|---|---|---|---|
| Amiodarone | Lidocaine | ||
| CYP3A62 | 0.008 ± 0.008 | 0.054 ± 0.012 | |
| CYP3A1 | 0.038 ± 0.005 | 0.081 ± 0.004 | |
| CYP3A2 | 0.018 ± 0.015 | 0.174 ± 0.006 | |
| CYP3A9 | 0.156 ± 0.010 | 0.178 ± 0.007 | |
| CYP3A18 | 0.084 ± 0.008 | 0.067 ± 0.006 | |