TABLE 1

Comparison of freshly isolated proximal tubule cells from 21-day STZ-diabetic and age-matched nondiabetic rats

Values are the mean ± S.E. of 15 cell isolation experiments.




Yield

% Viability

GGT Activity

Protein
106 cells dye exclusion nmol/min mg/106
Diabetic 32.7 ± 3.9 89.7 ± 2.2 257 ± 9 0.11 ± 0.04
Nondiabetic
32.6 ± 3.7
90.4 ± 1.1
251 ± 5
0.13 ± 0.05
  • GGT, γ glutamyltranspeptidase, a proximal tubular cell marker enzyme. There were no significant differences between diabetic and nondiabetic rat cells for any of the four values.