IC50 values of various diuretics for organic anion uptake mediated by hOAT1 and rOAT1
S2 hOAT1 was incubated in a solution containing either 5 μM [14C]PAH in the absence or presence of various concentrations of diuretics for 2 min at 37°C. Each value represents the mean ± S.E. of eight monolayers from two separate experiments.
Diuretic | hOAT1 | rOAT1a | |
---|---|---|---|
μM | |||
Acetazolamide | 75.0 ± 6.52 | 1100 | |
Bumetanide | 7.60 ± 0.58 | 1.9 | |
Furosemide | 18.0 ± 1.10 | 7.4 | |
Hydrochlorothiazide | 67.3 ± 5.32 | 265 |