TABLE 2

IC₅₀ values of various diuretics for organic anion uptake mediated by hOAT1, hOAT2, hOAT3, and hOAT4

S₂ hOAT1, S₂ hOAT2, S₂ hOAT3, and S₂ hOAT4 were incubated in a solution containing either 5 μM [¹⁴C]PAH (hOAT1), 50 nM [³H] PGF_2α (hOAT2), or 50 nM [³H]ES (hOAT3 and hOAT4) in the absence or presence of various concentrations of diuretics for 2 min (hOAT1, hOAT3, and hOAT4) or 20 s (hOAT2). Each value represents the mean ± S.E. of eight monolayers from two separate experiments.

Diuretics	hOAT1	hOAT2	hOAT3	hOAT4
	μM
Thiazide
Chlorothiazide	3.78 ± 0.41	2205 ± 143	65.3 ± 4.83	2632 ± 132
Cyclothiazide	84.3 ± 7.32	39.2 ± 4.10	27.9 ± 2.12	5000>
Hydrochlorothiazide	67.3 ± 5.32	1023 ± 53.2	942 ± 76.5	5000>
Trichloromethiazide	19.2 ± 1.12	1220 ± 101	71.2 ± 4.32	1505 ± 98.3
Loop diuretic
Bumetanide	7.60 ± 0.58	77.5 ± 5.38	0.75 ± 0.07	348 ± 23.2
Ethacrynic acid	29.6 ± 1.63	121 ± 9.83	0.58 ± 0.08	8.76 ± 0.91
Furosemide	18.0 ± 1.10	603 ± 46.3	7.31 ± 0.81	44.5 ± 2.53
Carbonic anhydrase inhibitor
Acetazolamide	75.0 ± 6.52	5000>	816 ± 67.3	425 ± 39.3
Methazolamide	438 ± 32.1	5000>	97.5 ± 9.84	5000>