TABLE 1

Bmax and Kd values from saturation binding experiments of [3H]DAMGO in HT, SC, and DRG membranes of animals with (24 and 96 h FCA) and without (control) inflammation

Membranes were incubated with varying concentrations of [3H]DAMGO as described under Materials and Methods. Data are mean values of at least four independent experiments, obtained from nonlinear regression analysis of binding saturation curves.



Control

FCA24 h

FCA96 h

Kd
Bmax
Kd
Bmax
Kd
Bmax
nM fmol / mg nM fmol / mg nM fmol / mg
HT 0.5 ± 0.2 578 ± 38 0.6 ± 0.1 561 ± 24 0.3 ± 0.1 530 ± 42
SC 0.5 ± 0.1 93 ± 14 0.8 ± 0.1 97 ± 7 0.8 ± 0.2 108 ± 7
DRG
0.2 ± 0.1
25 ± 1
0.6 ± 0.3
83 ± 10*
0.4 ± 0.1
49 ± 3*
  • * p < 0.05, significantly different from control animals