Effect of EP and GSH-Et on the release of NO and IL-6 by LPS-stimulated RAW 264.7 cells RAW 264.7 cells growing in multiwell plates were incubated with 10 ng/ml E. coli LPS for 24 h. Results are presented as means ± S.D. (n = 6 for all conditions except condition 2; n = 3 for condition 2).
Condition | First Preincubation (T = -75 to -15 min) | Second Preincubation (T = -15 to 0 min) | LPS (T = 0 to 24 h) | Nitrite | IL-6 |
|---|---|---|---|---|---|
| μM | pg / ml | ||||
| 1 | Medium | Medium | - | 8.4 ± 0.8* | 1 ± 1* |
| 2 | Medium | 6 mM GSH-Et | - | 8.1 ± 0.1 | 35 ± 5† |
| 3 | Medium | Medium | + | 83.4 ± 4.6 | 5145 ± 348 |
| 4 | 20 mM EP | Medium | + | 25.9 ± 1.1* | 298 ± 19* |
| 5 | Medium | 6 mM GSH-Et | + | 84.2 ± 3.4 | 5325 ± 314 |
| 6 | 20 mM EP | 6 mM GSH-Et | + | 34.6 ± 1.1‡* | 343 ± 11*‡ |