TABLE 1

Peptide analogs competing against 125I-hPP binding to HEK293 cells stably expressing the wild-type and GFP2-tagged rhY4 receptor

Eleven-point displacement curves were used to determine the affinity of various peptides or peptide analogs when competing with 125I-hPP binding to the wild-type or GFP2-tagged rhY4 receptor. The data were best fit to a one-site model, and Ki values (nM) for each inhibitor were determined using Prism software. Each value represents an average of four independent determinations performed in quadruplicate (mean ± S.E.M.).


Peptide and Peptide Analogs

rhY4 (WT) Receptor

rhY4-GFP2 Receptor
Human neuropeptide Y 0.323 ± 0.037 3.01 ± 0.26
Human peptide YY 0.202 ± 0.018 1.32 ± 0.21
Human [d-Trp32]NPY >100 >100
Human [Leu31,Pro34]NPY 0.261 ± 0.043 1.93 ± 0.26
Human NPY (3-36) 15.10 ± 1.78 >100
Human NPY (13-36) 11.72 ± 1.43 124.1 ± 4.61
Human pancreatic polypeptide 0.0243 ± 0.002 0.046 ± 0.011
1229U91
0.0344 ± 0.007
0.233 ± 0.104