TABLE 1

Primer sequences

Bold type indicates nucleic acid polymorphism.


PRIMERS UGT1A9

SEQUENCES

PRIMERS UGT1A7

SEQUENCES
PCR amplification PCR amplification
#37 5′-gtgctggtatttctccc #18 5′-cgctggacggcaccattg
#38 5′-gtcaaaaatgtcattgtatgaacc #17 5′-gctaaaggggagataacttacc
#39 5′-gatctggaccgggagttcaa #122 5′-gctggacggcaccattg
#40 5′-gtgtggctgtagagatcatact #123 5′-ccctaagagaagtctgggg
#41 5′-catgcacttggaggaacatttatta Allelic specific oligos
#42 5′-gagtacacgcattggcac G115 5′-catccaatggtattttt
Direct sequencing S115 5′-catccaatagtattttt
#7 5′-ctcccacctactgtatc Taqman analysis (codon 129/131)
#8 5′-gttcaaggcttttgccc #387 5′-gcaccattgcgaagtgcat
#9 5′-catttattatgccaccg #388 5′-ggatcgagaaacactgcatcaa
Allelic specific oligos N129/R131-FAM 5′-ttaatgaccgaaaatt
C3 5′-atggcttgcacagggt K129/K131-TET 5′-tttaaggacaaaaaatt
Y3 5′-atggcttacacagggt Taqman analysis (codon 139)
M33 5′-agtgcccatggatggga #546 5′-gcgaagtgcattttctctattaacaa
T33 5′-agtgcccacggatggga #544 5′-aagccacagcgatcaaaagg
Site-directed mutagenesis E139-Fam 5′-atacttaaaggagagttgttt
C3 to Y3 5′-gttctctgatggcttacacagggtggaccag D139-Vic 5′-atacttaaaggacagttgttt
5′-ctggtccaccctgtgtaagccatcagagaac Site-directed mutagenesis
M33 to T33 5′-gctactggtagtgcccacggatgggagccactgg E139 to D139 5′-aattagtagaatacttaaaggacagttgttttgatgcagtgtttc
5′-ccagtggctcccatccgtgggcactaccagtagc 5′-gaaacactgcatcaaaacaactgtcctttaagtattctactaatt
G115 to S115 5′-gttcatccaatagtatttttgac



5′-gtcaaaaatactattggatgaac