TABLE 1

DNA amplification primers

Amplicon Coordinates^a	Primer Sequence (5′ to 3′)			Exon	PCR Conditions^b
Amplicon Coordinates^a		Forward	Reverse	Exon	PCR Conditions^b
−10,443 to −10,003	CTTTTGGTTTTCCTGGTATG	GGAGTCTGATCTTGGGAAT		A
−10,078 to −9,573	TTCCAACATGACTCTGATAA	GCACAGTGGATAAACCTG		A
−9,647 to −9,413	AAATATAAACAACGGCTAACCT	AGACCCCACTTTCCTCAT	1	B
−137 to +153	GAGACCCGCTACAGAAGAT	AGTTGTTGAAACCCCACTT	2	C
+9,442 to +9,669	CACTGTTTGTACAGGAAC	AGAGAAAATAGGTAGGAAAT	3	C
+17,224 to +17,521	ACAACTGCTAATCATATCTG	CTATTTAATGGAACAGCC	4	C
+20,600 to +20,808	TCCTGTTAATTCTCTGTGTGAC	CACCCAGTAACATCAGGAA	5	C
+22,651 to +22,935	AGCCGTGTTCACAGTGTCA	TCTTCCTAAAAGCCCTTGG	6	C
+23,856 to +24,293	TGACAAGTCTCCTCCCTGTT	TCACATGGCACACCAAA	7	B
+24,943 to +25,153	TGAGTAGCTGGTACTAGACGTG	GGGTGCATTAAGTAGTTAACTTACC	8	B
+27,093 to +27,449	CCCCAATCTTCCTTTTATA	AAATCAGAAAAATAGCCACA	9	B
+27,380 to +27,648	CCATCTCCCTTTGAAAGTT	TCTAGCATTAGAAGTGGAGC	9	A
+27,595 to +27,928	CCATCTCCCTTTGAAAGTT	TCTAGCATTAGAAGTGGAGC	9	A

↵ a Coordinates are based on assigning the “A” of the ATG translation initiation codon +1.
↵ b A, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing at 50°C for 30 s, and elongation at 75°C for 90 s, and a final elongation at 75°C for 5 min; B, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing at 55°C for 30 s, and elongation at 75°C for 90 s, and a final elongation at 75°C for 5 min; C, denaturation at 94°C for 5 min followed by 30 cycles of denaturation at 92°C for 30 s, annealing and elongation at 55°C for 90 s, and a final elongation at 75°C for 5 min.