Binding affinities (Ki) of various ligands for NPFF receptors on membranes of rat spinal cord (rNPFF column), and CHO cells transfected with the human NPFF2 receptor (hNPFF2 column)
| The affinities of the ligands were determined in competition binding assays with 125I-(1DMe)Y8Fa, as described under Materials and Methods. Results are given as mean ± S.E.M. for a minimum of three experiments. | ||||
|---|---|---|---|---|
| Ligand | Ki | |||
| rNPFF | hNPFF2 | |||
| nM | ||||
| NPFF | 0.13 ± 0.03 | 5.2 ± 2.4 | ||
| (1DMe)Y8Fa | 0.20 ± 0.07 | 5.3 ± 1.0 | ||
| NPFF-OH | 2400 ± 700 | 4200 ± 800 | ||
| rNPSF | 5.2 ± 1.2 | 37 ± 12 | ||
| hNPY | 920 ± 170 | 4000 ± 1200 | ||
| hNPY18-36 | 860 ± 130 | 1200 ± 200 | ||
| BIBP3226 | 390 ± 50 | 640 ± 80 | ||
| hPrRP20 | 2.3 ± 0.5 | 23 ± 5 | ||
| hPrRP31 | 5.9 ± 2.0 | 19 ± 4 | ||
| hRFRP-1 | 15 ± 7 | 15 ± 2 | ||
| hRFRP-3 | 19 ± 3 | 55 ± 4 | ||
| FMRFamide | 2.2 ± 0.6 | 13 ± 2 | ||
| hPrRP24-31 | 11 ± 5 | 32 ± 5 | ||