Table 2
Michaelis-Menten kinetic parameters for the acyl glucuronidation of diclofenac in rat, dog, and human liver microsomes in the presence or absence of the membrane pore-forming peptide alamethicin (at a concentration of 25 μg/mg of microsomal protein)
| Species | Michaelis-Menten Kinetic Parameters for Acyl Glucuronidation of Diclofenac in Liver Microsomes | |||||
|---|---|---|---|---|---|---|
| Without Alamethicin | With Alamethicin | |||||
| Vmax | Km | CL
| Vmax | Km | CL
| |
| pmol/min/mg protein | μM | μl/min/mg protein | pmol/min/mg protein | μM | μl/min/mg protein | |
| Rat | 606 | 14 | 43.3 | 4,160 | 20 | 208 |
| Dog | 4,480 | 48 | 93.3 | 13,900 | 60 | 232 |
| Human | 2,660 | 18 | 148 | 11,500 | 18 | 639 |