IC50 values of NSAIDs and NO-NSAIDs in different cell lines
| Compound | IC50 at 48 h | ||||
|---|---|---|---|---|---|
| Prostate | Lung | Colon | Tongue | Pancreas | |
| μM | |||||
| Aspirin | 4820 ± 180 | 4090 ± 150 | > 50003-151 | > 50003-151 | > 50003-151 |
| NO-aspirin (NCX4040) | 0.8 ± 0.23-150 | 80 ± 53-150 | 2 ± 0.53-150 | 1.5 ± 0.33-150 | 7.5 ± 13-150 |
| Ratio | 6200 ± 470 | 51 ± 1 | > 2000 | > 2777 | > 588 |
| Sulindac | 1450 ± 100 | 75 ± 5 | 650 ± 50 | > 10003-151 | 800 ± 50 |
| NO-sulindac (NCX1102) | 92 ± 73-150 | 65 ± 33-150 | 28 ± 43-150 | 35 ± 53-150 | 65 ± 43-150 |
| Ratio | 16 ± 0.4 | 1 ± 0.1 | 23 ± 1 | > 25 | 12 ± 0.3 |
| Ibuprofen | > 10003-151 | > 10003-151 | > 10003-151 | > 10003-151 | 440 ± 30 |
| NO-ibuprofen (NCX2111) | 45 ± 43-150 | 35 ± 33-150 | 50 ± 43-150 | 79 ± 63-150 | 40 ± 43-150 |
| Ratio | > 20 | > 26 | > 19 | > 12 | 11 ± 0.4 |
Lung, pancreas (MIA PaCa-2), colon, prostate, and tongue cancer cell lines were treated with various concentrations of NO-aspirin, NO-sulindac, and NO-ibuprofen and their corresponding traditional NSAIDs, as described under Materials and Methods. Cell numbers were determined at 48 h from which IC50 values were calculated. Results are mean ± S.E.M. of three to seven different experiments done in duplicate.