Table 2

LightCycler PCR conditions for quantitative RT-PCR of transporter genes in mammary epithelial cells

Alternative Name	MgCl₂	Primer Conc	T_M	Cycle	Standard Tissue	Fluorescence Acquisition
	mM	μM	°C
MDR1	3	0.3	65	50	Kidney tissue	72°C^2-a
MRP1	3	0.3	64	50	ZR-75	72°C^2-a
MRP2	3	0.3	66	50	HepG2	72°C^2-a
MRP3	3	0.3	65	50	HepG2	72°C^2-a
MRP4	4	0.3	65	50	HT-29	87°C
MRP5	3	0.3	65	50	ZR-75	72°C^2-a
OAT1	3	0.3	64	50	Kidney tissue	72°C^2-a
OAT2	3	0.3	64	50	HepG2	72°C^2-a
OAT3	3	0.3	64	50	Kidney tissue	72°C^2-a
OAT4	3	0.3	65	50	Kidney tissue	72°C^2-a
OATP-A	3	0.3	66	50	ZR-75	72°C^2-a
OATP-B	3	0.3	64	50	HepG2	72°C^2-a
OATP-C	3	0.3	65	50	HT-29	72°C^2-a
OATP-D	3	0.3	65	50	ZR-75	72°C^2-a
OATP-E	3	0.25	64	50	HepG2	Melting to 98°C
OCT1	3	0.3	65	50	HepG2	72°C^2-a
OCT2	3	0.3	64	50	Kidney tissue	72°C^2-a
OCT3	3	0.3	64	50	HepG2	72°C^2-a
OCTN1	3	0.3	65	50	786-0	85°C
OCTN2	3	0.3	65	50	HepG2	72°C^2-a
CNT1	3	0.3	65	50	Kidney tissue	87°C
CNT2	3	0.3	65	50	HepG2	87°C
CNT3	3	0.3	65	50	HT-29	83°C
ENT1	3	0.3	64	50	ZR-75	72°C^2-a
ENT2	3	0.3	65	50	ZR-75	72°C^2-a
ENT3	3	0.3	64	50	786-0	72°C^2-a
NCBT1	3	0.3	64	50	ZR-75	72°C^2-a
NCBT2	3	0.3	65	50	Kidney tissue	87°C
PEPT1	3	0.3	66	50	HepG2	72°C^2-a
PEPT2	4	0.3	66	50	Kidney tissue	86°C
β-Actin	3	0.3	64	35	ZR-75	72°C^2-a
β-Casein	3	0.3	64	50	Milk cells^2-b	72°C^2-a
α-Lactalbumin	3	0.3	64	50	Milk cells^2-b	72°C^2-a

↵2-a Fluorescence acquisition at the end of the elongation phase.
↵2-b Cells isolated from fresh human breast milk provided positive control tissue for the calibration curve.