Table 1

Primer sequences and amplicon size (base pairs) for β-actin, β-casein, α-lactalbumin, and each transporter gene

Transporter Gene	Alternative Name	Forward Primer (5′–3′)	Reverse Primer (5′–3′)	Amplicon Size
ABCB1	MDR1	`TCACCTTCGTCAGCTACTTCGG`	`CAGGAGGTCACAGCCGACTTTAAAC`	698
ABCC1	MRP1	`CGGAAACCATCCACGACCCTAATC`	`ACCTCCTCATTCGCATCCACCTTG`	294
ABCC2	MRP2	`ATGGCAGTGAAGAAGAAGACGATGAC`	`GACGATGATGGTGAAGACAGGAGTGG`	755
ABCC3	MRP3	`AACTATGCCCCCGATGAGGACCAA`	`AGAGTGGAGATGGCGTTGAAGAAG`	752
ABCC4	MRP4	`CGGGCATACAAAGCAGAAGAGAGG`	`CAGTTCCTCATCCGTGTGCTCCTT`	696
ABCC5	MRP5	`ATCAAGCAAGGAAGCGGGAACACC`	`GCCACAAGGAACCACGGGAAGACT`	401
SLC22A6	OAT1	`CCACCTCTTCCTCTGCCTCTCCAT`	`GTCTGTTTCCCTTTCCTGCTCTCC`	573
SLC22A7	OAT2	`CTATCCCCAGGCTCTCCCCAACAC`	`GAAGCCATCGCCAGTCCCGTATCA`	530
SLC22A8	OAT3	`CGTCTTCTTCCTATCATCCTGGTG`	`CTGGCTCCTGCTTTGGCTTCTTTG`	905
SLC22A9	OAT4	`TGCCCTCTTGCTCAGTTTCCTT`	`CCTGGGCTGCTGTTGATTTCTG`	434
SLC21A3	OATP-A	`GACCTGACTGTTCCTTGATG`	`TGTATCTGAAGGTGGTGGAA`	291
SLC21A9	OATP-B	`CGCACTCACTGATTCCTACACATT`	`AAGCCATAGCATCACTCATTACAC`	278
SLC21A6	OATP-C	`GGTGAATGCCCAAGAGATGATG`	`TGAAGACAAGCCCAAGTAGACC`	327
SLC21A11	OATP-D	`TACGACAATGTGGTCTACCGATAC`	`CAGAGTTCAGCCCTCCTTTAGTCA`	455
SLC2112	OATP-E	`GCACCTCGGGCCTGTCCCGCTACC`	`GAGCAGGATGAACGTGGGGTTCTT`	423
SLC22A1	OCT1	`GATTTCCTTTACTCCGCTCTGGTC`	`TTTCTTTGGGCTTTGCTTTTCTCC`	487
SLC22A2	OCT2	`GATTTCTTCTACTCTGCCCTGGTT`	`GGATTTCTACTTTTGGTCTTGCTG`	599
SLC22A3	OCT3	`GACAAGAGAAGCCCCCAACCTGAT`	`CACTAAAGGAGAGCCAAAAATGTC`	455
SLC22A4	OCTN1	`AGGTGTGCTTCTCTTCATTCAACT`	`TCGTTACAGTCAGTCCATTTCAGT`	525
SLC22A5	OCTN2	`CTTTGCTGTTTGTCCTCTTGACCT`	`GCTCATTCTGCTCCATCTTCATTA`	474
SLC28A1	CNT1	`GCCTGCTGGGTGCCTACATCTCCT`	`TCCCACGCCACACCCATCAAGAAG`	385
SLC28A2	CNT2	`ACCTCTGCTCATCCGTCCCTACCTT`	`TCTCTCCCTCAATCCACTCCTCCAT`	602
SLC28A3	CNT3	`GCAGTGGATTATTAGAAAGGTTGG`	`ATGAAGATGTAGGAGCAGATTAGC`	549
SLC29A1	ENT1	`CTGGCTTTCTCTGTCTGCTTCATC`	`CCTCTGCTCCTGATGATGATGAAC`	518
SLC29A2	ENT2	`TACTGCTGCTCTTTGCCCTGAC`	`TTGAAGAGGAGGAAGCAGCAGA`	697
SLC29A3	ENT3	`ACAGAACCACAAGCAGCAGTCTCC`	`CCGTAGATGCTGCTGCTGAAGACA`	514
SLC23A1	NCBT1	`TTCCCTCCCGACAGCACAAAGT`	`AAAGCCACACACGCCCCCTACAA`	692
SLC23A2	NCBT2	`TCCGCATCCCCTACCCCTGTCA`	`GCACCCGCCCACAAACATCTCC`	611
SLC15A1	PEPT1	`GCCAGCACATACCAGTTTTT`	`TCCGCTGGGTTGATGTAAGT`	488
SLC15A2	PEPT2	`GATGATGAGGTGAAGGTGACAGTG`	`GAGGAATGTGCTTATCTGCTGGAC`	874
β-Actin		`ATCTGGCACCACACCTTCTACAATGAG`	`CGTCATACTCCTGCTTGCTGATCCACA`	837
β-Casein		`ATGAGGACCAGCAGCAAGGAGA`	`GGAGCAGAGGCAGAGGAAGATG`	318
α-Lactalbumin		`AGTTGTGAGTGTCTGCTGTCC`	`TCTGTGCTGTAGTGAAAGTGC`	241