Table 2

Effect of chronic cannabinoid administration on agonist-stimulated [³⁵S]GTPγS binding in mouse brain

Region	Net WIN-Stimulated [³⁵S]GTPγS Binding		Net Methanandamide-Stimulated [³⁵S]GTPγS Binding
Region	Vehicle	Δ⁹-THC	WIN55,212-2	Vehicle	Δ⁹-THC	WIN55,212-2
	fmol/mg		fmol/mg
Prefrontal cortex	5.54 ± 0.38	1.17 ± 0.14^2-a	1.97 ± 0.10^2-a ^2-c	2.01 ± 0.19	0.44 ± 0.07^2-a	0.59 ± 0.10^2-a
Nucleus accumbens	3.19 ± 0.30	0.48 ± 0.11^2-a	1.05 ± 0.18^2-a ^2-d
Cingulate cortex	5.18 ± 0.27	0.77 ± 0.10^2-a	1.47 ± 0.29^2-a ^2-d	1.64 ± 0.12	0.61 ± 0.12^2-a	0.74 ± 0.24^2-a
Caudate-putamen	4.14 ± 0.25	1.21 ± 0.17^2-a	1.69 ± 0.14^2-a ^2-d	1.64 ± 0.20	0.70 ± 0.07^2-b	0.91 ± 0.23^2-b
Globus pallidus	10.38 ± 0.35	7.18 ± 0.58^2-a	7.81 ± 0.49^2-a	3.42 ± 0.29	1.70 ± 0.25^2-a	1.99 ± 0.13^2-a
Hippocampus	3.52 ± 0.27	0.68 ± 0.18^2-a	1.17 ± 0.12^2-a ^2-d	1.22 ± 0.29	0.41 ± 0.09^2-a	0.52 ± 0.15^2-a
Amygdala	5.60 ± 0.33	1.11 ± 0.18^2-a	2.06 ± 0.24^2-a ^2-c
Hypothalamus	1.81 ± 0.27	0.19 ± 0.08^2-a	0.54 ± 0.19^2-a
Thalamus	0.74 ± 0.10	0.35 ± 0.09	0.45 ± 0.07
Entopeduncular nucleus	8.94 ± 0.59	5.97 ± 0.44^2-a	7.24 ± 0.38^2-d
Entorhinal cortex	5.69 ± 0.43	1.19 ± 0.24^2-a	1.76 ± 0.21^2-a
Substantia nigra	9.12 ± 0.29	6.40 ± 0.48^2-a	7.17 ± 0.33^2-a	3.72 ± 0.26	2.02 ± 0.25^2-a	2.29 ± 0.19^2-a
PAG	0.75 ± 0.09	0.17 ± 0.06^2-a	0.30 ± 0.14^2-b
Cerebellum	3.97 ± 0.33	1.45 ± 0.11^2-a	2.20 ± 0.14^2-a ^2-c	1.67 ± 0.17	0.52 ± 0.12^2-a	1.03 ± 0.12^2-a ^2-c

Sections were processed as described under Materials and Methods, and data represent mean net agonist-stimulated [³⁵S]GTPγS binding ± S.E.M. of triplicate sections from eight mice in each group.

↵2-a p < 0.01,
↵2-b p < 0.05 different from vehicle by Tukey-Kramer test;
↵2-c p < 0.01,
↵2-d p < 0.05 different from Δ⁹-THC by Tukey-Kramer test.