Effect of pregabalin (200 mg/kg s.c.) on the inflammatory parameters in the proximal colon at day 7
| Weight | MPO Content | Hyperemia and Necrosis | |
|---|---|---|---|
| g | U/mg of protein | % of colonic area | |
| Saline | 0.62 ± 0.02 (8) | 4.04 ± 0.94 (8) | 0.4 ± 0.1 (8) |
| TNBS | 1.39 ± 0.10 (6) | 34.45 ± 8.31 (6) | 31.1 ± 6.0 (6) |
| Pregabalin (200 mg/kg s.c.) | 1.50 ± 0.09 (8) | 43.87 ± 5.56 (8) | 28.4 ± 5.1 (8) |
One unit of MPO activity was defined as that which would produce an increase in absorbance (Δ A450 nm) of 1.0 unit/min at pH 7.0 and 25°C, calculated from the initial rate of reaction with peroxide (1 μM) as the substrate. The results are expressed as MPO units per milligram of protein (mean ± S.E.M.; n = 6–8/group).