Table 1

Primers used for PCR-SSCP analysis of the CYP2A13 coding region

Primer	5′ → 3′ Sequence	Location	Product Size (bp)
Exon 1F	tcaaccacagtccatccctc	−82 → −63
			410
Exon 1R	cctgtctttcctgctaggaccc	328 → 307
Exon 2F	cccagtacatgatatctcaggc	378 → 400
			294
Exon 2R	gagaccattgcatccacctga	671 → 651
Exon 3F	ttcacctccccaggcgtggc	1563 → 1582
			173
Exon 3R	ccctactcaccgtgcgtgcc	1735 → 1716
Exon 4F	cctgtaggcgccaatatcg	1926 → 1944
			162
Exon 4R	cgtggaggttgccgtgaact	2087 → 2068
Exon 5F	cctggacagatgcctttaactccg	3144 → 3167
			332
Exon 5R	tggctttgcacctgcctgcact	3475 → 3454
Exon 6F	gagagtgagcttggtctaaaccg	5122 → 5144
			205
Exon 6R	tcactttcctccctttccagt	5326 → 5306
Exon 7F	gtggaagctatgtcaaccg	5679 → 5696
			318
Exon 7R	tagtctgagtggtggtgg	5996 → 5979
Exon 8F	actcctccatgtcgtgccactcc	6368 → 6389
			306
Exon 8R	tgctggtgtgagccgtgg	6673 → 6656
Exon 9F	tcctcaggaaagcggtactg	7282 → 7301
			166
Exon 9R	tgtagtttcgtgggatcgtg	7447 → 7428

The forward (F) and reverse (R) primers for the amplification of each exon, plus exon-intron boundaries, are shown. The CYP2A13sequence from the completed human genome data base (accession no.AC008962) was used as a reference for documenting the location of the PCR primers, with the A of the ATG start codon (nucleotide 66807 inAC008962) designated as ⁺1.