Treatment | Activity % |
---|---|
Control | 100 |
1 μM inhibitor 2 | 48 ± 4.3 |
4 nM okadaic acid | 60.6 ± 3.8 |
1 mM cAMP | 102.8 ± 5.7 |
Activity was measured with 32P-labeled phosphorylase a in NRK cell extracts, pretreated with or without the drugs in vitro, and expressed as the percentage of phosphate released by untreated cell lysates (180–240 nmol/mg/h). Data are average of three to four experiments.