Effect of various in vitro treatments on the concentration-response curves to des-Arg9-BK in 5-h-incubated HUV
| Treatment | Period | pEC50 | Maximal response (g) | n | ||
|---|---|---|---|---|---|---|
| Control | Treated | Control | Treated | |||
| Bay 11-7082 (3 μM) | 0–5 h | 7.57 ± 0.13 | 7.31 ± 0.06 | 11.1 ± 1.5 | 9.4 ± 0.4 | 4 |
| Bay 11-7082 (10 μM) | 0–5 h | 7.32 ± 0.04 | 6.93 ± 0.06 | 16.6 ± 1.3 | 12.0 ± 1.71-150 | 6 |
| Bay 11-7082 (30 μM) | 0–5 h | 7.53 ± 0.19 | Not determined | 11.6 ± 2.0 | 0.3 ± 0.31-150 | 3 |
| Bay 11-7082 (10 μM) | 4.5–5 h | 7.32 ± 0.04 | 7.25 ± 0.05 | 16.6 ± 1.3 | 14.8 ± 0.8 | 6 |
| MG-132 (0.3 μM) | 0–5 h | 7.61 ± 0.07 | 7.53 ± 0.06 | 12.5 ± 1.6 | 11.2 ± 1.6 | 4 |
| MG-132 (1 μM) | 0–5 h | 7.37 ± 0.14 | 7.00 ± 0.11 | 14.6 ± 2.0 | 8.5 ± 2.71-150 | 7 |
| MG-132 (10 μM) | 0–5 h | 7.70 ± 0.13 | Not determined | 19.1 ± 2.4 | 7.0 ± 2.51-150 | 3 |
| MG-132 (1 μM) | 4.5–5 h | 7.42 ± 0.10 | 7.49 ± 0.09 | 14.8 ± 1.9 | 15.6 ± 2.5 | 7 |
| Interleukin-6 (10 ng/ml) | 0–3 h | 7.66 ± 0.07 | 7.93 ± 0.061-150 | 16.9 ± 2.2 | 15.7 ± 1.6 | 6 |
| Interleukin-4 (20 ng/ml) | 0–5 h | 7.72 ± 0.08 | 7.29 ± 0.121-150 | 14.0 ± 1.5 | 11.8 ± 1.5 | 5 |
| Interleukin-4 (20 ng/ml) | 4.5–5 h | 7.26 ± 0.12 | 7.50 ± 0.13 | 11.3 ± 1.0 | 12.0 ± 1.3 | 4 |
| TGF-β1 (3 ng/ml) | 0–5 h | 7.64 ± 0.04 | 7.23 ± 0.041-150 | 15.0 ± 1.4 | 12.7 ± 1.0 | 6 |
| TGF-β1 (3 ng/ml) | 4.5–5 h | 7.68 ± 0.08 | 7.56 ± 0.11 | 15.3 ± 3.2 | 14.0 ± 2.8 | 5 |
↵1-150 Significant differences between treated and paired control tissues (P < 0.05).